Résumé

Cold atmospheric pressure plasmas, which are partially ionized gases, are used for a variety of biomedical applications, such as the decontamination of surgical instruments. Recently, an alternative method has been developed that is easier to implement: the use of cold plasma-activated media (PAM). PAM treatment of skin wounds appears to promote healing. Thanks to the reactive oxygen and nitrogen species they contain, PAMs stimulate the migration of keratinocytes and fibroblasts, as well as angiogenesis, essential mechanisms for wound healing, while inhibiting bacterial proliferation. To overcome the limitations of traditional in vitro and in vivo models of skin wound healing, we developed a standardized ex vivo model using burned human skin explants, that preserves full skin architecture and enables detailed analysis of wound healing processes in a human-specific context. This reproducible superficial burn model allowed us to monitor wound healing over time, paving the way to assess and understand the molecular effects of PAM on wound repair