Résumé

Cold atmospheric plasma (CAP) is a promising therapeutic modality for the treatment of cholangiocarcinoma (CCA), offering selective cytotoxic effects through the generation of reactive oxygen and nitrogen species (RONS) and electric field. However, the existence of biological barriers (e.g. epithelial tissues) between CAP and CCA may reduce the penetration of plasma-generated species and therefore the therapeutic benefits of CAP therapy. In this study, RONS are generated using an atmospheric pressure plasma jet (APPJ) to treat the upper surface of various tissue models, while the presence of long-lived reactive species is assessed on the opposite (basal) side. Particular emphasis is placed on ex vivo murine skin samples, which are used to mimic in vivo barriers and to explore the trans-barrier transport of RONS. The ultimate objective is to identify key parameters that influence species penetration and to refine CAP t